Volume 11, Issue 4 (2-2025)
J Jiroft Univ Med Sci 2025, 11(4): 1768-1776 |
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Dortaj F, Masirzadeh M, Delkhosh A. The Evaluation of Oxidative Stress Status and Autophagy of Lung Tissue Following Nicotine Administration in Male Rats. J Jiroft Univ Med Sci 2025; 11 (4) :1768-1776
URL: http://journal.jmu.ac.ir/article-1-794-en.html
URL: http://journal.jmu.ac.ir/article-1-794-en.html
1- Graduate of Veterinary Medicine, Faculty of Veterinary Medicine, Tabriz Medical Sciences, Islamic Azad University, Tabriz, Iran
2- Assistant Professor, Department of Physiology, Faculty of Veterinary Medicine, Tabriz Medical Sciences, Islamic Azad University, Tabriz, Iran ,mr.nasirzadeh@iaut.ac.ir
3- Post Graduate of Pathology, Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
2- Assistant Professor, Department of Physiology, Faculty of Veterinary Medicine, Tabriz Medical Sciences, Islamic Azad University, Tabriz, Iran ,
3- Post Graduate of Pathology, Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Abstract: (249 Views)
Introduction: The relationship between reactive oxygen species and autophagy is critical for cell homeostasis and survival. Today, it is known that oxidative stress and autophagy play a key role in the pathogenesis of various diseases. The present study has investigated the state of oxidative stress in lung tissue and autophagy after receiving nicotine.
Materials and Methods: In this study, 30 male rats weighing 190±20 grams were randomly selected and divided into 3 groups (n=10). Control group: intact healthy mice, group two: daily intake of nicotine with a dose of 0.25 mg/Kg and group three: daily intake of nicotine with a dose of 0.5 mg/Kg, mice received nicotine intraperitoneally for 30 days. At the end of the study, activity of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GPX), total antioxidant capacity (TAC) and Malondialdehyde (MDA) level of lung tissue were measured. In addition, tissue changes and P62 and LC3 markers of autophagy process were evaluated.
Results: The results of the study showed that nicotine administration caused a significant increase in MDA and a significant decrease in the activity of antioxidant enzymes SOD and GPX. Also, the results related to lung tissue changes show that nicotine administration has increased fibrosis and stimulated the autophagy process. Conclusion: This study showed that nicotine stimulates the process of autophagy in the lung tissue of male rats by causing oxidative stress. |
Type of Study: Research |
Subject:
Medical Sciences / Physiology
Received: 2024/07/27 | Accepted: 2025/01/18 | Published: 2025/02/28
Received: 2024/07/27 | Accepted: 2025/01/18 | Published: 2025/02/28
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