Volume 3, Issue 2 (2-2017)                   J Jiroft Univ Med Sci 2017, 3(2): 23-38 | Back to browse issues page

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Bayat H, Naderi F, Mohammadian O, Rahimpour A. The CRISPR-Cas system and Recent Advances in Its Precision Performance. J Jiroft Univ Med Sci 2017; 3 (2) :23-38
URL: http://journal.jmu.ac.ir/article-1-122-en.html
The CRISPR-Cas system and Recent Advances in Its Precision Performance
Hadi Bayat1 , Fatemeh Naderi2 , Omid Mohammadian3 , Azam Rahimpour 4
1- Department of Tissue Engineering and Regenerative Medicine, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2- Department of Genetics, School of Advanced Sciences, Islamic Azad University, Tehran Medical Branch, Tehran, Iran
3- Clinical Biochemistry, Departments of Clinical Biochemistry, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4- Department of Tissue Engineering and Regenerative Medicine, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran , rahimpour@sbmu.ac.ir
Abstract:   (4736 Views)

Introduction: Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR associated (CRISPR-Cas) system is an adaptive immune system present in the most of bacteria and archaea. During the recent years, CRISPR-Cas has been introduced as a genome editing technology with high compatibility to different laboratory conditions in eukaryotic cell.

Methods: In the present review study, recent advances of this system and key points with regard to improving its efficiency in research and clinical application will be covered. In this regard, keywords including CRISPR-Cas, efficiency improvement, and off target have been analyzed in PubMed and 72 titles were selected and used in this paper. 

Results: CRISPR-Cas system is widely used for studying the function of genomic loci, development of engineered organisms as animal models and also clinical research of genetic diseases in stem cells due to the simplicity of its design. In this regard, improving the performance and precision of this system is necessary for the accomplishment of the efficient and safe genetic manipulation.

Conclusion: Different approaches have been used for improving the function of CRISPR-Cas system including optimization of the design, target recognition, binding and cutting of the target sites.

Keywords: CRISPR-Cas, DNA repair, double-stranded DNA break
Full-Text [PDF 804 kb]   (6476 Downloads)    
Type of Study: Research | Subject: Medical Sciences / Medical Genetics
Received: 2016/12/18 | Accepted: 2016/12/29 | Published: 2017/06/21
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