fa تاثیر تمرین مقاومتی و نانوکورکومین بر نشانگرهای التهابی بافت تومور (NF-κB و IL-1α) در موش های صحرایی مبتلا به گلیوبلاستومای مولتی فرم علوم پزشکی / فيزيولوژي Medical Sciences / Physiology پژوهشي Research <div> <table align="left" hspace="0" vspace="0"> <tbody> <tr> <td style="padding: 0cm 12px; text-align: justify;" valign="top"><span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">مقدمه و هدف:</span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> هدف از مطالعه حاضر بررسی تأثیر تمرین مقاومتی و مکمل نانوکورکومین بر مقادیر پروتئینی </span></span>NF-&kappa;B<span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> و </span></span>IL-1&alpha;<span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> درگیر در متابولیسم تومور در موش&shy;های صحرایی مبتلا به گلیوبلاستومای مولتی فرم (</span></span>GBM<span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">) می&shy; باشد.</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra""></span></span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">روش کار:</span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">30 سر موش صحرایی نر ویستار به 5 گروه کنترل سالم،</span></span>GBM <span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">، </span></span>GBM<span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">+ تمرین مقاومتی (</span></span>RT<span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">)، </span></span>GBM<span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">+ مکمل نانوکورکومین (</span></span>NCUR<span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">) و </span></span>GBM+RT+NCUR<span style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> تقسیم شدند. </span></span>GBM<span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> در قشر فرونتال موش‌ها تزریق شد. گروه تمرینی برای مدت 4 هفته، 3 روز در هفته،</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">تمرینات مقاومتی فزاینده را انجام دادند. در پایان، موش‌ها قربانی شدند و تغییرات بافتی تومور مغزی با روش </span></span>H&E<span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> و همچنین مقادیر پروتئینی </span></span>NF-&kappa;B<span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> و </span></span>&nbsp;IL-1&alpha;<span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">با روش وسترن بلات ارزیابی شدند.</span></span><b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra""></span></span></b></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">یافته&shy; ها:</span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> نسبت به گروه کنترل سالم گروه </span></span>GBM<span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> تغییرات معنی &shy;دار بافتی (افزایش مساحت تومور) و افزایش مقادیر پروتئینی </span></span>&nbsp;IL-1&alpha;<span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">را نشان داد (</span></span>p<<span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Mitra""></span></span><span style="font-size:12.0pt"><span b="" mitra="" style="font-family:">0/05</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">).<b> </b>در بررسی تغییرات بافتی مشخص شد که گروه&shy;های </span></span>GBM+RT<span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">، </span></span>GBM+NCUR<span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> و </span></span>GBM+RT+NCUR<span style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> (</span></span>p<<span style="font-size:12.0pt"><span b="" mitra="" style="font-family:">0/05</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">) کاهش معنی&shy; داری را در مساحت تومور مغز را نسبت به گروه </span></span>GBM<span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> نشان دادند. همچنین گروه </span></span>GBM+RT+NCUR<span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> کاهش معنی&shy; دار در مساحت تومور را نسبت به گروه&shy; های </span></span>GBM+RT<span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> و </span></span>GBM+NCUR<span style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> (</span></span>p<<span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Mitra""></span></span><span style="font-size:12.0pt"><span b="" mitra="" style="font-family:">0/05</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">) نشان داد. </span></span><b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra""></span></span></b></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">نتیجه‌گیری:</span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> به نظر می &shy;رسد برای ارزیابی تغییرات نشانگرهای التهابی درگیر در متابولیسم تومور باید از مدت زمان درمانی بیشتر، دوز متفاوت مکمل مصرفی، تمرینات ورزشی متفاوت، شدت و حجم تمرین ورزشی متنوع استفاده کرد. همچنین پیشرفت بیماری می &shy;تواند از عوامل تأثیرگذار بر عدم تغییر فاکتورهای مولکولی مطالعه حاضر باشد.</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra""></span></span></span></span></span></span></span></span></td> </tr> </tbody> </table> </div> <div style="text-align: justify;"></div> <div> <table align="center" hspace="0" vspace="0"> <tbody> <tr> <td align="left" style="padding-top:0cm; padding-right:12px; padding-bottom:0cm; padding-left:12px" valign="top"> <pre style="text-align: justify;"> <span style="font-size:10pt"><span style="background:white"><span style="text-justify:inter-ideograph"><span courier="" new="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Introduction</span></b><b><span new="" roman="" style="font-family:" times="">:</span></b><span new="" roman="" style="font-family:" times=""> The aim of this study was to investigate the effect of resistance training and nanocurcumin supplementation on protein amounts of NF-</span><span cambria="" math="" style="font-family:">&kappa;</span><span new="" roman="" style="font-family:" times="">B and IL-1</span><span cambria="" math="" style="font-family:">&alpha;</span><span new="" roman="" style="font-family:" times=""> involved in tumor metabolism in rats with glioblastoma multiforme (GBM).</span><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></pre> <div style="text-align: justify;"><span style="font-size:10pt"><span style="background:white"><span style="line-height:normal"><span new="" roman="" style="font-family:" times=""><b>Materials </b><b>and Methods</b>:30 male Wistar rats were divided into 5 groups namely, healthy control, GBM, GBM+resistance training (RT), GBM+nanocurcumin supplement (NCUR), and GBM+RT+NCUR. GBM was injected into the frontal cortex of rats. The training group performed incremental resistance training on the ladder for 4 weeks, 3 days per week. At the end, the rats were sacrificed and the histological changes of the brain tumor were evaluated by H&E method, and also the expression of NF-<span cambria="" math="" style="font-family:">&kappa;</span>B and IL-1<span cambria="" math="" style="font-family:">&alpha;</span> protein were measured by western blot method.</span></span></span></span><br> <span style="font-size:10pt"><span style="background:white"><span style="line-height:normal"><span new="" roman="" style="font-family:" times=""><b>Results:</b> Compared to the healthy control group, the GBM group showed significant tissue changes (increasing tumor area) and increased IL-1<span cambria="" math="" style="font-family:">&alpha;</span> protein levels (p<span cambria="" math="" style="font-family:">&le;</span>0/05). In the analysis of tissue changes, it was found that the GBM+RT, GBM+NCUR and GBM+RT+NCUR (p<span cambria="" math="" style="font-family:">&le;</span>0/05) groups showed a significant reduction in the brain tumor area compared to the GBM group. Also, the GBM+RT+NCUR group showed a significant decrease in tumor area compared to the GBM+RT and GBM+NCUR groups (p<span cambria="" math="" style="font-family:">&le;</span>0/05).</span></span></span></span><br> <span style="font-size:10pt"><span style="background:white"><span style="text-justify:inter-ideograph"><span style="line-height:normal"><span new="" roman="" style="font-family:" times=""><b>Conclusion:</b> It seems that, to evaluate the changes of inflammatory markers involved in tumor metabolism, longer treatment duration, different dosage of supplements, different exercise training, intensity and volume of exercise training should be used. Also, the progress of the disease can be one of the factors affecting the non-change of the molecular factors of the present study.</span></span></span></span></span></div> </td> </tr> </tbody> </table> </div> <div style="text-align: justify;"></div> تومور, مغز, تمرین مقاومتی, کورکومین, مکمل نانو Tumor, Brain, Resistance training, Curcumin, Nano supplement 1460 1470 http://journal.jmu.ac.ir/browse.php?a_code=A-10-910-1&slc_lang=fa&sid=1 Mehdi Begham مهدی بی غم 10031947532846008102 10031947532846008102 No PhD Student of Exercise Physiology, Aras International Campus, University of Tehran, Tehran, Iran دانشجوی دکتری تخصصی فیزیولوژی ورزشی، واحد پردیس بین‌الملل ارس، دانشگاه تهران، تهران، ایران Fatemeh Shabkhiz فاطمه شب خیز shabkhiz@ut.ac.ir 10031947532846008103 10031947532846008103 Yes Associate Professor, Exercise Physiology Department, Faculty of Sport Sciences and Health, University of Tehran, Tehran, Iran دانشیار گروه فیزیولوژی فعالیت ورزشی، دانشکده علوم ورزشی و تندرستی، دانشگاه تهران، تهران، ایران Hossein Shirvani حسین شیروانی 10031947532846008104 10031947532846008104 No Associate Professor of Exercise Physiology, Exercise Physiology Research Center, Lifestyle Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran دانشیار فیزیولوژی ورزشی، مرکز تحقیقات فیزیولوژی ورزش، پژوهشکده سبک زندگی، دانشگاه علوم پزشکی بقیه‌الله، تهران، ایران Mosa Khalafi موسی خلفی 10031947532846008105 10031947532846008105 No Assistant Professor of Exercise Physiology, Department of Sports Sciences, Faculty of Humanities, University of Kashan, Kashan, Iran استادیار فیزیولوژی ورزشی، گروه علوم ورزشی، دانشکده علوم انسانی، دانشگاه کاشان، کاشان، ایران