fa تاثیر تمرین هوازی و بربرین کلراید بر بیان ژن‌های SIRT1 و AMPK بافت چربی احشایی موش‌های صحرایی دیابتی علوم پزشکی / پزشکي ورزشي Medical Sciences / Sports Medicine پژوهشي Research <div> <table align="left" hspace="0" vspace="0"> <tbody> <tr> <td style="padding: 0cm 12px; text-align: justify;" valign="top"><span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">مقدمه و هدف:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">فرو</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">پاشی میتوکندری ناشی از استرس اکسیداتیو یکی از عوامل مهم مقاومت به انسولین است. هدف از پژوهش حاضر بررسی اثر تمرین هوازی و بربرین کلراید بر شاخص‌های بازسازی و بیوژنز میتوکندری بافت چربی احشایی موش‌های صحرایی دیابتی با استرپتوزوتوسین می&shy; باشد.</span></span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">روش کار:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">32 موش صحرایی نر ویستار به طور تصادفی به چهار گروه (8=</span></span><span dir="LTR" style="font-size:12.0pt">n</span><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">): دیابت</span></span><span b="" mitra="" style="font-family:"> (</span>DM<span b="" mitra="" style="font-family:">)</span><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">، دیابت-بربرین </span></span><span b="" mitra="" style="font-family:">(</span>BDM<span b="" mitra="" style="font-family:">)</span><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">، دیابت-تمرین هوازی </span></span><span b="" mitra="" style="font-family:">(</span>TDM<span b="" mitra="" style="font-family:">)</span><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">، دیابت-تمرین هوازی-بربرین </span></span><span b="" mitra="" style="font-family:">(</span>TBDM<span b="" mitra="" style="font-family:">)</span><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> تقسیم شدند. دیابت با تزریق </span></span>STZ <span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">در موش‌های نر القا شد. بربرین کلراید ( 30</span></span>mg/kg/day<span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">) یک بار در روز به صورت خوراکی با گاواژ خورانده شد. گروه‌های تمرین به مدت شش هفته برنامه تمرین هوازی فزاینده (18-10 متر در دقیقه، 40-10 دقیقه در روز، پنج روز در هفته) را روی تردمیل انجام دادند. سطوح بیان ژن فاکتورهای </span></span>SIRT1 <span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">و </span></span>AMPK <span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">بافت چربی احشایی با استفاده از روش </span></span>Real time-PCR<span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> اندازه&shy; گیری شد. تجزیه و تحلیل داده &shy;ها با استفاده از آزمون آنالیز واریانس یکطرفه و در صورت معناداری از آزمون تعقیبی توکی در سطح معنی‌داری 0/05</span></span>>p<span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> استفاده شد.</span></span><b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra""></span></span></b></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">یافته &shy;ها:</span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">تجزیه و تحلیل داده‌ها نشان داد که اختلاف معنی‌داری در بیان ژن&shy;های </span></span>SIRT1<span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> (</span></span>p<<span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">0/05</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">) و </span></span>AMPK<span style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> (</span></span>p<<span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Mitra""></span></span><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">0/05</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">) در بین گروه&shy;ها وجود دارد.</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">در ادامه نتایج نشان داد که بیان ژن </span></span>SIRT1<span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> در گروه دیابت-تمرین (</span></span>p<<span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Mitra""></span></span><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">0/05</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">) و دیابت-تمرین-بربرین‌کلراید <a name="_Hlk155617450">(</a></span></span>p<<span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Mitra""></span></span><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">0/05</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">) افزایش معنی‌داری نسبت به گروه دیابت داشت. بربرین‌کلراید به تنهایی نتوانست موجب افزایش معنی‌دار بیان ژن </span></span>SIRT1<span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> در مقایسه با گروه دیابتی شود <a name="_Hlk155613455">(</a></span></span>p><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Mitra""></span></span><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">0/05</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">)</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">.</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">همچنین بیان ژن </span></span>AMPK<span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> در گروه دیابت-تمرین (</span></span>p<<span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Mitra""></span></span><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">0/05</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">)، دیابت-بربرین&shy; کلراید (</span></span>p<<span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Mitra""></span></span><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">0/05</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">) و دیابت-تمرین-بربرین‌کلراید (</span></span>p<<span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Mitra""></span></span><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">0/05</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">) افزایش معنی‌داری نسبت به گروه دیابت داشت</span></span><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">.</span></span></span></span></span></span></span></span></td> </tr> </tbody> </table> </div> <div style="text-align: justify;"><b><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:110%"><span b="" mitra="" style="font-family:">نتیجه‌گیری:</span></span></span></b> <span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:110%"><span b="" mitra="" style="font-family:">براساس یافته&shy; های فوق احتمال دارد تاثیر همزمان تمرین و بربرین کلراید بتواند استرس اکسیداتیو و التهاب ناشی از دیابت را از طریق مسیر </span></span></span><span style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">AMPK-SIRT1-PPAR&gamma;</span></span></span><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:110%"><span b="" mitra="" style="font-family:"> مهار کند.</span></span></span></div> <div> <table align="center" hspace="0" vspace="0"> <tbody> <tr> <td align="left" style="padding-top:0cm; padding-right:12px; padding-bottom:0cm; padding-left:12px" valign="top"> <pre style="text-align: justify;"> <span style="font-size:10pt"><span style="background:white"><span style="text-justify:inter-ideograph"><span courier="" new="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Introduction</span></b><b><span new="" roman="" style="font-family:" times="">:</span></b> <span new="" roman="" style="font-family:" times="">Mitochondrial collapse caused by oxidative stress is one of the important factors of insulin resistance. The purpose of this study is to investigate the effect of aerobic exercise and berberine chloride on the indices of mitochondrial regeneration and biogenesis of visceral adipose tissue in streptozotocin-diabetic rats.</span><b><span style="font-family:"Times New Roman","serif""></span></b></span></span></span></span></pre> <div style="text-align: justify;"><span style="font-size:10pt"><span style="background:white"><span style="text-justify:inter-ideograph"><span style="line-height:normal"><span new="" roman="" style="font-family:" times=""><b>Materials </b><b>and Methods</b><b>:</b> 32 male Wistar rats were randomly divided into four groups (n=8): diabetes (DM), diabetes-berberine (BDM), diabetes-aerobic exercise (TDM), diabetes-aerobic exercise-berberine (TBDM). Diabetes was induced by STZ injection in male rats. Berberine chloride (30 mg/kg/day) was administered orally by gavage once a day. The exercise groups performed an incremental aerobic exercise program (10-18 m/min, 10-40 min/day, and five days/week) on a treadmill for six weeks. Gene expression levels of SIRT1 and AMPK factors in visceral adipose tissue were measured using Real time-PCR method. Data analysis was conducted using one-way analysis of variance and if significant, Tukey&#39;s post hoc test was used at a significance level of p<0.05.</span></span></span></span></span><br> <span style="font-size:10pt"><span style="background:white"><span style="text-justify:inter-ideograph"><span style="line-height:normal"><span new="" roman="" style="font-family:" times=""><b>Results:</b> Data analysis showed that there is a significant difference in the expression of SIRT1 (p<0.05) and AMPK (p<0.05) genes among the groups. Furthermore, the results showed that the expression of SIRT1 gene in the diabetes-exercise group (p<0.05) and diabetes-exercise-berberine chloride (p<0.05) had a significant increase compared to the diabetes group. Berberine chloride alone could not significantly increase SIRT1 gene expression compared to the diabetic group (p>0.05). Also, the expression of AMPK gene in diabetes-exercise (p<0.05), diabetes-berberine chloride (p<0.05) and diabetes-exercise-berberine chloride (p<0.05) groups had a significant increase compared to the diabetes group.</span></span></span></span></span><br> <span style="font-size:10pt"><span style="background:white"><span style="text-justify:inter-ideograph"><span style="line-height:normal"><span new="" roman="" style="font-family:" times=""><b>Conclusion:</b> Based on the above findings, it is possible that the simultaneous effect of exercise and berberine chloride could inhibit oxidative stress and inflammation caused by diabetes through the AMPK-SIRT1-PPAR&gamma; pathway.</span></span></span></span></span></div> </td> </tr> </tbody> </table> </div> <div style="text-align: justify;"></div> تمرین هوازی, بربرین کلراید, بیوژنز میتوکندری, دیابت Aerobic exercise, Berberine chloride, Mitochondrial biogenesis, Diabetes 1348 1356 http://journal.jmu.ac.ir/browse.php?a_code=A-10-475-1&slc_lang=fa&sid=1 sajjad Asghari Vostakolaei سجاد اصغری وسطی کلایی 10031947532846007583 0009-0005-9710-1098 No Assistant Professor, Department of Physical Education and Sport Sciences, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran استادیار، گروه تربیت بدنی وعلوم ورزشی، واحد قائمشهر، دانشگاه آزاد اسلامی، قائمشهر، ایران Saqqa Farajtabar Behrestaq سقا فرج تبار بهرستاق farajtabarp@yahoo.com 10031947532846007584 10031947532846007584 Yes MA of Physical Education, Department of Physical Education and Sport Sciences, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran کارشناسی ارشد تربیت بدنی،گروه تربیت بدنی وعلوم ورزشی، واحد قائمشهر، دانشگاه آزاد اسلامی، قائمشهر، ایران Babisan Askari بابی سان عسکری 10031947532846007585 10031947532846007585 No Assistant Professor, Department of Physical Education and Sport Sciences, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran استادیار، گروه تربیت بدنی وعلوم ورزشی، واحد قائمشهر، دانشگاه آزاد اسلامی، قائمشهر، ایران