fa اثرات تمرینات هوازی و عصاره زعفران بر بیان ژن‌های Bax، Bcl-2 و Caspase-3 در بافت هیپوکامپ موش‌های صحرایی نر آلزایمری علوم پزشکی / پزشکي ورزشي Medical Sciences / Sports Medicine پژوهشي Research <div> <table align="left" hspace="0" vspace="0"> <tbody> <tr> <td style="padding: 0cm 12px; text-align: justify;" valign="top"><span style="font-size:10pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">مقدمه و هدف:</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> با توجه به شیوع بیماری آلزایمر که رایج &shy;ترین شکل زوال عقل است و عدم وجود درمان قطعی برای آن، هدف از پژوهش حاضر بررسی اثر تمرین هوازی به همراه مصرف عصاره زعفران بر بیان ژن&shy; های </span></span>Bax<span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">، </span></span>Bcl-2<span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> و </span></span>Caspase-3 <span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">در موش&shy; های نر آلزایمری بود.</span></span><b><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Mitra""></span></span></b></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">روش کار:</span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> در</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">مطالعه</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">تجربی</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">حاضر،</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">48</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">موش نر بالغ</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">به</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">صورت</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">تصادفی</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">به 6 گروه کنترل، آلزایمر، آلزایمر و ورزش هوازی، آلزایمر و ورزش هوازی و عصاره، آلزایمر و عصاره و همچنین گروه کنترل و ورزش هوازی تقسیم شدند. مدل آلزایمر با تزریق آمیلوئید بتا </span></span><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">42-1 در </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">هیپوکمپ ایجاد شد.</span></span><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> تمرینات هوازی شامل 3 جلسه تمرین در هفته به مدت 8 هفته انجام شد. تعیین میزان بیان ژن&shy; های موردنظر از روش </span></span>Real Time PCR<span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> استفاده</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">گردید.</span></span><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> جهت تجزیه و تحلیل داده &shy;ها از آزمون آنالیز واریانس یک طرفه و از آزمون تعقیبی تام هن استفاده شد.</span></span><b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra""></span></span></b></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">یافته &shy;ها:</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> نتایج نشان داد بعد از 8 هفته تمرین هوازی بیان ژن </span></span>Bax <span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">در گروه</span></span><span dir="LTR" style="font-size:12.0pt">&shy;</span><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">های مداخله نسبت به گروه آلزایمر کاهش معنی</span></span><span dir="LTR" style="font-size:12.0pt">&shy;</span><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">دار یافت (0/05</span></span><span b="" mitra="" style="font-family:">></span>p<span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">). همچنین </span></span>Caspase-3 <span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">در گروه آلزایمر و ورزش هوازی و گروه آلزایمر و ورزش هوازی و عصاره نسبت به گروه آلزایمر کاهش معنی</span></span><span dir="LTR" style="font-size:12.0pt">&shy;</span><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">دار داشتند (0/05</span></span><span b="" mitra="" style="font-family:">></span>p<span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">). بیان ژن </span></span>Bcl-2<span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> در گروه آلزایمر و ورزش هوازی و گروه آلزایمر و ورزش هوازی و عصاره نسبت به گروه آلزایمر افزایش معنی</span></span><span dir="LTR" style="font-size:12.0pt">&shy;</span><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">داری را نشان دادند (0/05</span></span><span b="" mitra="" style="font-family:">></span>p<span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">).</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra""></span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">نتیجه&shy; گیری:</span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">به</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">نظر</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">می&shy; رسد</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">تمرین</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">هوازی</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">و</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">مصرف</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">عصاره</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">زعفران</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">موجب تغییر در بیان ژن&shy; های دخیل در آپوپتوز</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">در</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">موش صحرایی </span></span><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">نر آلزایمری می&shy; گردد. </span></span><span dir="LTR" style="font-size:12.0pt"></span></span></span></span></span></span></span></span></td> </tr> </tbody> </table> </div> <div style="text-align: justify;"></div> <div> <table align="center" hspace="0" vspace="0"> <tbody> <tr> <td style="padding: 0cm 12px; text-align: justify;" valign="top"><span style="font-size:10pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times=""><b><span style="line-height:110%">Introduction:</span></b> <span style="line-height:110%">Considering the prevalence of Alzheimer&#39;s disease, which is the most common form of dementia, and the lack of a definitive treatment for it, the purpose of this study is to investigate the effect of exercise training with the consumption of saffron extract on the expression of Bax, Bcl-2, and Caspase-3 genes in male rats with Alzheimer&#39;s disease.</span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times=""><b><span style="line-height:110%">Materials </span></b><b><span style="line-height:110%">and Methods</span></b><b><span style="line-height:110%">:</span></b> <span style="line-height:110%">In this experimental study, 48 adult male rats were randomly divided into 6 groups: control, Alzheimer, Alzheimer and endurance training, Alzheimer and endurance training and extract, Alzheimer and extract, and the control and endurance training group. The Alzheimer&#39;s model was created by injecting amyloid beta 42-1 into the CA1 region of the hippocampus. Aerobic exercises, including 3 training sessions per week, were performed on alternate days for 8 weeks. The Real-Time PCR method was used to determine the expression level of the desired genes. Two-way analysis of variance and Tomhane&#39;s post hoc test were used to analyze the data.</span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:normal"><span new="" roman="" style="font-family:" times=""><b>Results:</b> &nbsp;The results showed that after 8 weeks of aerobic training, the Bax gene expression in the intervention groups significantly decreased compared to the Alzheimer&#39;s group (p<0.05). Also, Caspase-3 in Alzheimer and endurance training, and Alzheimer and endurance training and extract group had a significant decrease compared to Alzheimer&#39;s group (p<0.05). Bcl-2 gene expression in Alzheimer and endurance training, and Alzheimer and endurance training and extract showed a significant increase compared to Alzheimer&#39;s group (p<0.05).</span></span></span><br> <b><span style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></span></b> <span style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">It seems that aerobic exercise and consumption of saffron extract improve the factors involved in apoptosis in Alzheimer&#39;s male mice.</span></span></span></td> </tr> </tbody> </table> </div> <div style="text-align: justify;"></div> تمرینات ورزشی, زعفران, Bcl-2, Caspase-3, آلزایمر Exercise, Saffron extract, Bcl-2, Caspase-3, Alzheimer 1151 1159 http://journal.jmu.ac.ir/browse.php?a_code=A-10-746-2&slc_lang=fa&sid=1 Fazel Bazyar فاضل بازیار 10031947532846007854 10031947532846007854 No Ph.D. Candidate of Exercise Physiology, Department of Physical Education and Sport Science, Faculty of Humanities, Rasht Branch, Islamic Azad University, Rasht, Iran دانشجوی دکترای فیزیولوژی ورزشی، گروه تربیت بدنی و علوم ورزشی، دانشکده علوم انسانی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران Ramin Shabani رامین شعبانی dr.ramin.shabani@gmail.com 10031947532846007855 10031947532846007855 Yes Associate Professor, Faculty of Humanities, Department of Physical Education and Sports Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran استاد فیزیولوژی ورزشی، گروه تربیت بدنی و علوم ورزشی، دانشکده علوم انسانی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران Alireza Elmiyeh علیرضا علمیه 10031947532846007856 10031947532846007856 No Assistant Professor, Faculty of Humanities, Department of Physical Education and Sports Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran دانشیار، گروه تربیت بدنی و علوم ورزشی، دانشکده علوم انسانی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران