Journal of Jiroft University of Medical Sciences
مجله دانشگاه علوم پزشکی جیرفت
J Jiroft Univ Med Sci
Medical Sciences
http://journal.jmu.ac.ir
1
admin
2538-2810
2538-2810
10.61882/jjums
fa
jalali
1401
5
1
gregorian
2022
8
1
9
2
online
1
fulltext
fa
اثر یک دوره تمرین هوازی همراه با مکمل یاری پروبیوتیک بر بیان ژن های SIRT-1و FNDC-5 در بطن چپ موش های نر دیابتی
The effect of an aerobic exercise training with complementary probiotic supplementation on the gene expression of SIRT-1 and FNDC-5 in the left ventricle of diabetic male rats
علوم پزشکی / پزشکي ورزشي
Medical Sciences / Sports Medicine
پژوهشي
Research
<div>
<table align="left" hspace="0" vspace="0">
<tbody>
<tr>
<td style="padding: 0cm 12px; text-align: justify;" valign="top"><span style="font-size:10pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Mitra"">مقدمه و هدف:</span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Mitra""> تمرین هوازی همراه با مصرف مکمل پروبیوتیک با تنظیم بیان ژن، ظرفیت آنتی­ اکسیدانی را در بطن چپ بیماران مبتلا به دیابت بهبود می ­دهد. هدف از این مطالعه، تعیین اثر یک دوره تمرین هوازی همراه با مکمل یاری پروبیوتیک بر</span></span><b> </b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra"">بیان ژن­ های </span></span> SIRT-1<span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra"">و</span></span>FNDC-5<span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra""> در بطن چپ موش ­های آزمایشگاهی نر مبتلا</span></span> <span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra"">به دیابت القاء شده با استرپتوزوتوسین بود.</span></span><b><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Mitra""></span></span></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Mitra"">روش کار:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra"">در پژوهش تجربی حاضر، 30 سر موش نر دیابتی به پنج گروه 6 تایی تقسیم شدند</span></span>;<span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra""> کنترل سالم </span></span><b>(</b>NC<b>)</b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra"">، دیابتی </span></span><b>(</b>DC<b>)</b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra"">، مکمل</span></span><b> </b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra"">دیابتی </span></span><b>(</b>DS<b>)</b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra"">، تمرین دیابتی (</span></span>DT<span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra"">)، تمرین دیابتی با مکمل </span></span><b> </b>.<b>(</b>DTS<b>) </b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra"">القاء دیابت به همه گروه ­ها جز کنترل سالم با تزریق درون صفاقی</span></span><b> </b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra"">استرپتوزوتوسین (</span></span>STZ<span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra"">) با دوز 60 میلی­ گرم بر هر کیلوگرم از وزن بدن بعد از 12 ساعت ناشتایی شبانه انجام شد. جهت تعیین بیان ژن­ های</span></span><b> </b>SIRT-1<span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra""> و </span></span>FNDC-5<span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra""> از روش </span></span>PCR- Real time<span style="font-size:12.0pt"><span style="font-family:"B Mitra""> استفاده شد و مقایسه گروه ­ها توسط آنووای یک طرفه در سطح آلفای 0/05 انجام شد. </span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Mitra"">یافته ­ها:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra"">بیان ژن</span></span>SIRT-1 <span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra""> در گروه­ های </span></span>DT<span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra""> و </span></span>DTS<span style="font-size:12.0pt"><span style="font-family:"B Mitra""> نسبت به گروه </span></span>DC<span style="font-size:12.0pt"><span style="font-family:"B Mitra""> به ترتیب<b> </b>(0/001</span></span><span dir="LTR" style="font-family:"Century Gothic","sans-serif""><</span>p<span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra"">) و</span></span><span style="font-size:12.0pt"><span style="font-family:"B Mitra"">(0/01</span></span><span dir="LTR" style="font-family:"Century Gothic","sans-serif""><</span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra""></span></span>p<span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra"">)</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra""> و بیان ژن </span></span>FNDC-5<span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra""> در گروه ­های</span></span>DT <span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra"">و </span></span>DTS<span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra""> نسبت به گروه </span></span>DC<b> </b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra"">به ترتیب </span></span><span style="font-size:12.0pt"><span style="font-family:"B Mitra"">(0/05</span></span><span dir="LTR" style="font-family:"Century Gothic","sans-serif""><</span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra""></span></span>p<span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra"">) و </span></span><span style="font-size:12.0pt"><span style="font-family:"B Mitra"">(0/001</span></span><span dir="LTR" style="font-family:"Century Gothic","sans-serif""><</span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra""></span></span>p<span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra"">) افزایش معناداری داشتند.</span></span> <span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra""></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Mitra"">نتیجه ­گیری:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra"">تمرین هوازی همراه با مکمل پروبیوتیک با تأثیر بر تنظیم بیان ژن ­</span></span> FNDC-5<span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra""> در بطن چپ موش­ های آزمایشگاهی دیابتی، احتمالاً میتواند دفاع ضد اکسایشی را افزایش داده و کاردیومیوپاتی دیابتی را بهبود دهد.</span></span><b><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Mitra""></span></span></b></span></span></span></span></span></td>
</tr>
</tbody>
</table>
</div>
<div style="text-align: justify;"></div>
<div>
<table align="center" hspace="0" vspace="0">
<tbody>
<tr>
<td style="padding: 0cm 12px; text-align: justify;" valign="top"><span style="font-size:10pt"><span style="line-height:110%"><span style="font-family:"Times New Roman","serif""><b><span style="line-height:110%">Introduction:</span></b><span lang="EN" style="line-height:110%"> Aerobic exercise with probiotic supplementation improves the antioxidant capacity in the left ventricle of diabetic patients by regulating gene expression.</span><span style="line-height:110%"> The purpose of this study was to investigate the </span><span lang="EN" style="line-height:110%">effect of an aerobic exercise</span><span style="line-height:110%"> training</span><span lang="EN" style="line-height:110%"> with complementary probiotic supplementation on the </span><span style="line-height:110%">gene expression of SIRT-1</span><span lang="EN" style="line-height:110%"> and FNDC-5 in the left ventricle </span><span style="line-height:110%">of streptozotocin-induced diabetic male rats.</span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:110%"><span style="font-family:"Times New Roman","serif""><b><span style="line-height:110%">Materials </span></b><b><span style="line-height:110%">and Methods</span></b><b><span style="line-height:110%">:</span></b><span style="line-height:110%"> In this experimental research, 30 diabetic male laboratory rats were divided into five groups of six including, non-diabetic control (NC), diabetic control (DC), diabetic supplement (DS), diabetic training (DT), diabetic training supplement (DTS). Diabetes was induced in all groups except for non-diabetic control group by </span><span lang="EN" style="line-height:110%">intraperitoneal injection of streptozotocin (STZ) at a dose of 60 mg per kilogram of body weight after 12 hours of overnight fasting.</span> <span lang="EN" style="line-height:110%">Real-time PCR technique was used to evaluate the gene expression of the</span><span style="line-height:110%"> SIRT-1and FNDC-5 and the </span><span lang="EN" style="line-height:110%">comparison of groups </span><span style="line-height:110%">was used</span><span lang="EN" style="line-height:110%"> by</span> <span lang="EN" style="line-height:110%">One way ANOVA at the alpha level of 0.05.</span><span style="line-height:110%"></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:110%"><span style="font-family:"Times New Roman","serif""><b><span style="line-height:110%">Results:</span></b> <span lang="EN" style="line-height:110%">SIRT-1</span> <span lang="EN" style="line-height:110%">gene expression in DT and DTS groups compared to DC group, (p<0.001) and (p<0.01) respectively, and </span><span style="line-height:110%">FNDC-5 gene expression in DT and DTS groups compared to DC group </span><span lang="EN" style="line-height:110%">(p<0.05) and (p<0.001)</span><span style="line-height:110%"> significantly increased</span><b><span style="line-height:110%"></span></b></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:normal"><span style="font-family:"Times New Roman","serif""><b>Conclusion:</b> Aerobic exercise combined with probiotic supplementation can possibly increase the antioxidant defense and improve diabetic cardiomyopathy by affecting the regulation of FNDC-5 gene expression in the left ventricle of diabetic rats.</span></span></span></td>
</tr>
</tbody>
</table>
</div>
<div style="text-align: justify;"></div>
تمرین هوازی, SIRT-1,FNDC-5, مقاومت به انسولین, مکمل پروبیوتیک
Aerobic exercise training, SIRT-1, FNDC-5, Insulin resistance, Probiotic supplement
999
1009
http://journal.jmu.ac.ir/browse.php?a_code=A-10-676-3&slc_lang=fa&sid=1
Seyedeh Somayeh
Samaie
سیده سمیه
سمایی
10031947532846006368
10031947532846006368
No
Ph.D Student in Sport Physiology, Alborz Campus, University of Tehran, Tehran, Iran
دانشجوی دکترای فیزیولوژی ورزشی، پردیس البرز دانشگاه تهران، تهران، ایران
Ali Asghar
Ravasi
علی اصغر
رواسی
aaravasi@ut.ac.ir
10031947532846006367
10031947532846006367
Yes
Professor, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran
استاد، گروه فیزیولوژی ورزش، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران
Sirous
Choobineh
سیروس
چوبینه
choobineh@ut.ac.ir
10031947532846006370
10031947532846006370
No
Associate Professor, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran
دانشیار، گروه فیزیولوژی ورزش، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران
Maryam
Delfan
مریم
دلفان
m.delfan@alzahra.ac.ir
10031947532846006369
10031947532846006369
No
Assistant Professor, Department of Exercise Phyiology, Faculty of Sport Sciences, Alzahra University, Tehran, Iran
استادیار، گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه الزهرا (س)، تهران، ایران