fa تأثیر دو نوع شدت تمرین تناوبی بر بیان ژن NRF1 و NRF2 میتوکندریایی کاردیومیوسیت‌ موش‌های نر مدل سکته قلبی علوم پزشکی / پزشکي ورزشي Medical Sciences / Sports Medicine پژوهشي Research <div> <table align="left" hspace="0" vspace="0"> <tbody> <tr> <td style="padding: 0cm 12px; text-align: justify;" valign="top"><span style="font-size:10pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">مقدمه و هدف: </span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">بیوژنز میتوکندری یک چرخه پیچیده است که شامل هماهنگی بین بیا</span></span><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">ن </span></span><span dir="LTR" style="font-size:12.0pt">&shy;</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">ژ</span></span><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">ن&shy;</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">های میتوکندری و ژن&shy; های هسته&shy; ای، سپس ورود محصولات به اندامک و تداوم گردش این چرخه می&shy;باشد</span></span><span dir="LTR" style="font-size:12.0pt">.</span><span style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> هدف از پژوهش حاضر تعیین ت</span></span><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">أ</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">ثیر دو نوع شدت تمرین تناوبی بر بیان ژن</span></span>NRF1 <span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">&nbsp;و </span></span>NRF2<span style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> میتوکندریایی کاردیومیوسیت‌ موش‌های نر مدل سکته قلبی بود</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">.</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra""></span></span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">روش کار:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">در این مطالعه تجربی</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">24</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> سر موش</span></span><b> </b><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">نر</span></span><b> </b><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">مدل</span></span><b> </b><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">سکته</span></span><b> </b><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">قلبی</span></span><b> </b><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">مواجهه</span></span><b> </b><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">با</span></span><b> </b><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">ایزوپروترنول </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">به &shy;صورت تصادفی به سه گروه تمرین تناوبی با شدت بالا (</span></span>HIIT<span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">)</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">، تمرین تناوبی با شدت متوسط (</span></span>MIIT<span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">)</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> و کنترل (</span></span>C<span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">) تقسیم</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> شدند. گروه&shy; های تمرین به مدت 8 هفته تحت </span></span><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">تا</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">ثیر تمرین تناوبی قرار گرفتند. تمرین در دو گروه شدت بالا و متوسط </span></span><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">با</span></span><b> </b><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">مسافت</span></span><b> </b><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">طی</span></span><b> </b><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">شده</span></span><b> </b><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">همسان</span></span><b> </b><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">شامل اجرای 10 مرحله فعالیت 4 دقیقه&shy; ای با شدت 90-85 و 70-65 درصد </span></span>VO₂max<span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> بود و در این زمان گروه کنترل برنامه تمرینی نداشت. بیان ژن</span></span>NRF1 <span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">&nbsp;و </span></span>NRF2<span style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> میتوکندریایی </span></span><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">با</span></span><b> </b><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">استفاده</span></span><b> </b><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">از</span></span><b> </b><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">روش </span></span>Real Time PCR <span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">به&shy; دست آمد. </span></span><b><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Mitra""></span></span></b></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">یافته &shy;ها:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">تفاوت معنی&shy; داری در </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">بیان ژن</span></span>NRF1 <span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">&nbsp;و </span></span>NRF2 <span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">میتوکندریایی کاردیومیوسیت‌ موش‌های نر مدل سکته قلبی</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">در سه گروه تمرین و کنترل مشاهده شد </span></span>(p&le;0/001). <span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">نتایج آزمون توکی بیانگر افزایش معنی&shy; دار </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">بیان ژن</span></span>NRF1 <span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">&nbsp;و </span></span>NRF2<span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> در دو گروه تمرین تناوبی نسبت به گروه کنترل </span></span><span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">بود </span></span>(p&le;0/001)<span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">. با این حال تمرینات </span></span>HIIT<span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:"> اثرات بیشتری داشت.</span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">نتیجه &shy;گیری:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">به&shy; طور</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">کلی به نظر می&shy;رسد که تمرینات تناوبی با شدت بالا و متوسط،</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" mitra="" style="font-family:">به افزایش بیوژنز میتوکندری عضله قلبی منجر می&shy; شوند.</span></span></span></span></span></span></span></td> </tr> </tbody> </table> </div> <div style="text-align: justify;"></div> <div> <table align="center" hspace="0" vspace="0"> <tbody> <tr> <td align="left" style="padding-top:0cm; padding-right:12px; padding-bottom:0cm; padding-left:12px" valign="top"><span style="font-size:10pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times=""><b><span style="line-height:110%">Introduction:</span></b><span style="line-height:110%"> Mitochondrial biogenesis is a complex cycle that involves the coordination between the expression of mitochondrial genes and nuclear genes, and then the entry of products into the organelle and the continuity of the circulation of this cycle. The purpose of this study was to</span><span lang="EN" style="line-height:110%"> investigate</span><span style="line-height:110%"> the effect of two types of interval training intensity on mitochondrial NRF1 and NRF2 mitochondrial gene expression in male rats&rsquo; myocardial infarction models</span><b><span style="line-height:110%"></span></b></span></span></span><br> <span style="font-size:10pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times=""><b><span style="line-height:110%">Materials </span></b><b><span style="line-height:110%">and Methods</span></b><b><span style="line-height:110%">:</span></b> <span style="line-height:110%">In this experimental study, 24 male stroke rats exposed to isoproterenol, were randomly divided into three groups: high-intensity intermittent exercise (HIIT), moderate-intensity intermittent exercise (MIIT), and control (C). Exercise groups were influenced by periodic exercise for 8 weeks. Exercise in the two groups of high and medium intensity, with the same distance traveled, included 10-minute activities with an intensity of 85-90 and 70-65% VO2max.;at this time the control group did not have a training program. Mitochondrial NRF1 and NRF2 gene expression was obtained by real-time PCR.</span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:normal"><span new="" roman="" style="font-family:" times=""><b>Results:</b> Significant differences were observed in the expression of mitochondrial NRF1 and NRF2 genes in cardiomyocytes of male myocardial infarction rats<b> </b>in three training and control groups (p&ge; 0.001). The results of Tukey test showed a significant increase in NRF1 and NRF2 gene expression in the two interval training groups compared to the control group (p&ge; 0.001). However, HIIT had more effects</span></span></span><br> <span style="font-size:10pt"><span style="line-height:normal"><span new="" roman="" style="font-family:" times=""><b>Conclusion:</b> In general, HIIT and MIIT appear to increase myocardial mitochondrial biogenesis..</span></span></span></td> </tr> </tbody> </table> </div> <span style="font-size:10.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times="">&nbsp; </span></span></span> انفارکتوس قلبی, بیوژنز میتوکندری, تمرین تناوبی با شدت بالا و تمرین تناوبی با شدت متوسط Myocardial infarction, Mitochondrial biogenesis, HIIT.MIIT 896 904 http://journal.jmu.ac.ir/browse.php?a_code=A-10-673-1&slc_lang=fa&sid=1 Mojdeh Nadaf fahmideh مژده نداف فهمیده 10031947532846006149 10031947532846006149 No PhD Student in Sports Physiology, Department of Physical Education and Sports Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran دانشجوی دکتری تخصصی فیزیولوژی ورزشی، گروه تربیت بدنی و علوم ورزشی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران Shahram Gholamrezaei شهرام غلامرضائی gholamrezaei@iaurasht.ac.ir 10031947532846006150 10031947532846006150 Yes Assistant Professor, PhD in Sports Physiology, Department of Physical Education and Sports Sciences, Rasht Branch, Islamic Azad University, Rasht , Iran استادیار، دکتری فیزیولوژی ورزشی، گروه تربیت بدنی و علوم ورزشی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران Ramin Shabani رامین شعبانی 10031947532846006151 10031947532846006151 No Professor, PhD in Sports Physiology, Department of Physical Education and Sports Science, Rasht Branch, Islamic Azad University, Rasht, Iran استاد، دکتری فیزیولوژی ورزشی، گروه تربیت بدنی و علوم ورزشی،واحد رشت ، دانشگاه آزاد اسلامی، رشت، ایران