fa مطالعه اثرات ضد مخمری میکروامولسیون عصاره گیاه عدس‌الملک بر سویه‌های کاندیدا علوم پزشکی / ميکرب شناسي Medical Sciences / Microbiology پژوهشي Research <table align="left" cellpadding="0" cellspacing="0" hspace="0" vspace="0"> <tbody> <tr> <td style="padding: 0in 9pt; vertical-align: top; text-align: justify;"><strong><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">مقدمه و هدف:</span></span></strong><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;"> عفونت‌های</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">کاندیدایی</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">دسته</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">بزرگی</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">از</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">بیماری&shy; ها</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">را</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">تشکیل</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">می&shy; دهند و امروزه</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">نیاز</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">به</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">مواد ضد قارچی کم ضرر،</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">بیشتر</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">از هر زمان دیگر احساس</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">می&shy; شود.</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">گیاه عدس &shy;الملک با</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">نام</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">علمی </span></span></span>&nbsp;<span dir="LTR"><em>securidaca (L.)</em></span><em><span dir="LTR">Securigera&nbsp;</span></em><span dir="LTR"> <em>&nbsp;</em></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">گیاهی</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">دارویی</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">با</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">خواص</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">ضد</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">میکروبی،</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">ضد </span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">آفت،</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">ضد انگلی</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">و</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">ضد</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">قارچی</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">است</span></span></span><span dir="LTR"><span style="color:black;">.</span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;"> هدف</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">از</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">انجام</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">این</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">مطالعه تعیین</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">اثرات</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">ضد</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">کاندیدایی</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">میکروامولسیون</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">عصاره &shy;های اتانولی و متانولی</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">گیاه</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">عدس &shy;الملک</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">بر</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">سویه &shy;های مختلف کاندیدا</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">می‌باشد</span></span></span><span dir="LTR"><span style="color:black;">.</span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;"></span></span></span><br> <strong><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">روش کار:</span></span></strong><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;"> در</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">این</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">مطالعه</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">آزمایشگاهی،</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">از عصاره&shy; های اتانولی و متانولی</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">گیاه</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">عدس &shy;الملک</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">و</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">توئین 80 برای ساخت میکروامولسیو</span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">ن </span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">استفاده</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">شد. پس از بررسی مشخصات فیزیکوشیمیایی (ویسکوزیته و </span></span></span><span dir="LTR"><span style="color:black;">pH</span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">)</span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">، روش چاهک برای بررسی اثر ضد مخمری فرمولاسیون بر روی سویه &shy;های کاندیدا به&shy; کار برده شد.</span></span></span><strong><span style="font-family:B Mitra;"><span style="font-size:12.0pt;"></span></span></strong><br> <strong><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">یافته&shy; ها:</span></span></strong><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;"> میکروامولسیون</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">عصاره‌ متانولی</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">گیاه</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">عدس &shy;الملک در</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">غلظت</span></span></span>&nbsp;<span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">0/01%</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">خواص ضد کاندیدایی قابل توجهی نشان داد. این میکروامولسیون‌ بر علیه کاندیدا آلبیکنس بیشترین اثر مهاری را داشت.</span></span></span><span style="font-family:B Mitra;"><span style="font-size:12.0pt;"></span></span><br> <strong><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">نتیجه&shy; گیری:</span></span></strong> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">میکروامولسیون‌های</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">سنتزشده</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">بر</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">پایه عصاره‌</span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">های</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">گیاهی</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">عدس &shy;الملک</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">دارای</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">پایداری</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">مناسب</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">بودند</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">و</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">خواص</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">ضد مخمر</span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">ی </span></span></span><span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">بالایی</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">را</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">علیه</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">سویه&shy; های کاندیدا</span></span></span> <span style="color:black;"><span style="font-family:B Mitra;"><span style="font-size:12.0pt;">نشان دادند.</span></span></span><br> &nbsp;</td> </tr> </tbody> </table> <div style="text-align: justify;"></div> <table align="center" cellpadding="0" cellspacing="0" hspace="0" vspace="0"> <tbody> <tr> <td style="text-align: justify;"><strong>Introduction:</strong> Candida infections constitute a large group of diseases, so today the need for low-harm antifungal agents is felt more than ever. <em>Securigera Securidaca L.</em> is a medicinal plant with antimicrobial, anti-pest, anti-parasitic and anti-fungal properties. Therefore, the aim of this study was to determine the anti-candida effects of microemulsion of ethanolic and methanolic extracts of Securigera Securidaca on different strains of Candida.<br> <strong>Materials </strong><strong>and Methods</strong><strong>:</strong> In this laboratory study, ethanolic and methanolic extracts of Securigera Securidaca and twin 80 were used to make microemulsions. After examining the physicochemical characteristics (viscosity and pH), the well diffusion method was used to investigate the anti-yeast effect of the formulation on Candida strains.<br> <strong>Results:</strong> Microemulsions in concentrations of 0.01% of Securigera Securidaca extracts showed significant anti-candidal properties. These microemulsions had the highest inhibitory effect against Candida albicans. <span dir="RTL"></span><br> <strong>Conclusion:</strong> Synthesized microemulsions based on Securigera Securidaca plant extract have good stability and showed high anti-yeast properties against Candida strains.</td> </tr> </tbody> </table> <div style="text-align: justify;"><div style="clear:both;"></div></div> خواص ضد مخمری, میکروامولسیون, عصاره عدس‌الملک, سورفاکتنت Anti-yeast, Microemulsion, Securigera Securidaca extract, Surfactant 690 698 http://journal.jmu.ac.ir/browse.php?a_code=A-10-585-1&slc_lang=fa&sid=1 Maryam Shirani مریم شیرانی 10031947532846005561 10031947532846005561 No Assistant Professor, Toxicology Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Medical Sciences, Ahvaz, Iran استادیار، مرکز تحقیقات سم‌شناسی، پژوهشکده علوم پایه پزشکی، دانشگاه علوم پزشکی جندی شاپور اهواز، اهواز، ایران Masoud Mahdavinia مسعود مهدوی نیا mahdavimasoud@yahoo.com 10031947532846005562 10031947532846005562 Yes Assistant Professor, Department of Toxicology, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran استادیار، گروه سم‌شناسی، دانشکده داروسازی، دانشگاه علوم پزشکی جندی شاپور اهواز، اهواز، ایران saeed khoshnood سعید خشنود 10031947532846005563 10031947532846005563 No Assistant Professor, Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran استادیار، مرکز تحقیقات میکروب شناسی بالینی، دانشگاه علوم پزشکی ایلام، ایلام، ایران Heibatullah Kalantari هیبت اله کلانتری 10031947532846005564 10031947532846005564 No Professor, Department of Pharmacology and Toxicology, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran استاد گروه سم شناسی، دانشکده داروسازی، دانشگاه علوم پزشکی جندی شاپور اهواز، اهواز، ایران Atefeh Raesi Vanani عاطفه رئیسی وانانی 10031947532846005565 10031947532846005565 No PhD in Toxicology, Department of Pharmacology and Toxicology, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran دکترای سم شناسی، گروه سم شناسی، دانشکده داروسازی، دانشگاه علوم پزشکی جندی شاپور اهواز، اهواز، ایران