fa بررسی ارتباط پلی‌مورفیسم‌های Gly972Arg و Ala512Pro در ژن IRS1 با بیماری دیابت نوع 2 در استان سیستان و بلوچستان علوم پزشکی / ژنتيک پزشکي Medical Sciences / Medical Genetics پژوهشي Research <strong><span style="font-size:14px;"><span style="font-family:iranyekan;"><span style="color:#0000CD;"><span dir="RTL">مقدمه:</span> </span></span></span><span dir="RTL"><span style="font-family:b lotus;"><span style="font-size:11.0pt;">دیابت نوع 2 شایع‌ترین نوع دیابت بوده و 90</span></span></span><span dir="RTL"><span style="font-family:times new roman,serif;"><span style="font-size:11.0pt;">٪</span></span></span><span dir="RTL"><span style="font-family:b lotus;"><span style="font-size:11.0pt;"> موارد این بیماری را به خود اختصاص داده است. ژن انسولین رسپتور سوبسترای -1 (</span></span></span><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">IRS1</span></span><span dir="RTL"><span style="font-family:b lotus;"><span style="font-size:11.0pt;">) به</span></span></span><span dir="RTL"><span style="font-family:b lotus;"><span style="font-size:11.0pt;">‌</span></span></span><span dir="RTL"><span style="font-family:b lotus;"><span style="font-size:11.0pt;">عنوان میانجی بین گیرنده</span></span></span><span style="font-family:calibri,sans-serif;"><span style="font-size:11.0pt;">‌</span></span><span dir="RTL"><span style="font-family:b lotus;"><span style="font-size:11.0pt;">های انسولین و شبکه پیچیده</span></span></span><span style="font-family:calibri,sans-serif;"><span style="font-size:11.0pt;">‌</span></span><span dir="RTL"><span style="font-family:b lotus;"><span style="font-size:11.0pt;">ای از مولکول‌های پیام</span></span></span><span style="font-family:calibri,sans-serif;"><span style="font-size:11.0pt;">‌</span></span><span dir="RTL"><span style="font-family:b lotus;"><span style="font-size:11.0pt;">رسان داخل سلول‌های ماهیچه</span></span></span><span style="font-family:calibri,sans-serif;"><span style="font-size:11.0pt;">‌</span></span><span dir="RTL"><span style="font-family:b lotus;"><span style="font-size:11.0pt;">ای، کبد و بافت چربی ایفای نقش می‌کند. لذا ممکن است کاندید مناسبی برای استعداد ابتلاء به دیابت نوع 2 باشد. در این مطالعه ارتباط پلی‌مورفیسم</span></span></span><span style="font-family:calibri,sans-serif;"><span style="font-size:11.0pt;">‌</span></span><span dir="RTL"><span style="font-family:b lotus;"><span style="font-size:11.0pt;">های </span></span></span><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">Gly972Arg</span></span><span dir="RTL"><span style="font-family:b lotus;"><span style="font-size:11.0pt;"> و </span></span></span><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">Ala512Pro</span></span><span dir="RTL"><span style="font-family:b lotus;"><span style="font-size:11.0pt;"> ژن </span></span></span><em><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">IRS1</span></span></em><span dir="RTL"><span style="font-family:b lotus;"><span style="font-size:11.0pt;"> با استعداد ابتلا</span></span></span><span dir="RTL"><span style="font-family:b lotus;"><span style="font-size:11.0pt;">ء به دیابت نوع 2، مورد ارزیابی قرار گرفت.</span></span><br> <span style="font-family:iranyekan;"><span style="color:#0000CD;"><span style="font-size:14px;">روش کار:</span></span></span></span></strong><span style="font-family:iranyekan;"><span style="color:#0000CD;"><span style="font-size:14px;"> </span></span></span><strong><span dir="RTL"><span style="font-family:b lotus;"><span style="font-size:11.0pt;">در این پژوهش مورد- شاهدی 100 بیمار مبتلاء به دیابت نوع 2 و 100 فرد سالم بررسی شدند. استخراج </span></span></span><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">DNA</span></span><span dir="RTL"><span style="font-family:b lotus;"><span style="font-size:11.0pt;"> با استفاده از روش ترکیب فنل- کلروفرم با پروتیناز </span></span></span><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">K</span></span><br> <span dir="RTL"><span style="font-family:b lotus;"><span style="font-size:11.0pt;">انجام شد. تعیین ژنوتیپ افراد به روش </span></span></span><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">RFLP-PCR</span></span><span dir="RTL"><span style="font-family:b lotus;"><span style="font-size:11.0pt;"> انجام شد. سپس آنالیز آماری نتایج با استفاده از آزمون کای-دو صورت گرفت.</span></span><br> <span style="font-family:iranyekan;"><span style="color:#0000CD;"><span style="font-size:12.0pt;">نتایج:</span></span></span></span> <span dir="RTL"><span style="font-family:b lotus;"><span style="font-size:11.0pt;">نتایج به‌دست آمده، نشان داد درصد فراوانی ژنوتیپ</span></span></span><span style="font-family:calibri,sans-serif;"><span style="font-size:11.0pt;">‌</span></span><span dir="RTL"><span style="font-family:b lotus;"><span style="font-size:11.0pt;">های </span></span></span><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">GG</span></span><span style="font-family:times new roman,serif;"><span style="font-size:11.0pt;">, </span></span><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">GA</span></span><span dir="RTL"><span style="font-family:b lotus;"><span style="font-size:11.0pt;"> و </span></span></span><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">AA</span></span><span dir="RTL"><span style="font-family:b lotus;"><span style="font-size:11.0pt;"> برای پلی‌مورفیسم </span></span></span><span style="font-family:times new roman,serif;"><span style="font-size:11.0pt;">(</span></span><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">Gly972Arg</span></span><span style="font-family:times new roman,serif;"><span style="font-size:11.0pt;">) </span></span><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">rs1801278</span></span><span dir="RTL"><span style="font-family:b lotus;"><span style="font-size:11.0pt;"> در افراد بیمار 18/82 و 0 درصد و در افراد سالم 85/15 و0 درصد بود. نتایج بررسی آماری نشان داد که فراوانی آللی و ژنوتیپی </span></span></span><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">Gly972Arg</span></span><span dir="RTL"><span style="font-family:b lotus;"><span style="font-size:11.0pt;"> در گروه بیمار و کنترل اختلاف معناداری نداشت (61/0=</span></span></span><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">P</span></span><span dir="RTL"><span style="font-family:b lotus;"><span style="font-size:11.0pt;">). همچنین پلی‌مورفیسم </span></span></span><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">Ala512Pro</span></span><span dir="RTL"><span style="font-family:b lotus;"><span style="font-size:11.0pt;"> در جمعیت سیستان و بلوچستان یافت نشد.</span></span><br> <span style="color:#0000CD;"><span style="font-size:14px;"><span style="font-family:iranyekan;">نتیجه‌گیری:</span></span></span></span></strong><span style="color:#0000CD;"> </span><strong><span dir="RTL"><span style="font-family:b lotus;"><span style="font-size:11.0pt;">تحلیل آماری نتایج به‌دست آمده، نشان‌دهنده عدم ارتباط آلل‌های این دو پلی‌مورفیسم از ژن </span></span></span><em><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">IRS1</span></span></em><span dir="RTL"><span style="font-family:b lotus;"><span style="font-size:11.0pt;"> در جمیعت سیستان و پلوچستان با خطر بروز بیماری دیابت نوع 2 بود</span></span></span><span dir="RTL"><span style="font-family:b mitra;"><span style="font-size:10.0pt;">.</span></span></span></strong><br> &nbsp; <hr><strong><span dir="LTR" style="background:aqua;"><span style="font-family:wingdings 3;"><span style="font-size:14.0pt;">…</span></span></span></strong> <strong><span style="background:aqua;"><span style="font-family:b roya;"><span style="font-size:12.0pt;">لطفاً به مقاله به شکل زیر استناد کنید:</span></span></span></strong><br> <strong><span style="font-family:b roya;"><span style="font-size:11.0pt;">اسلامی ع، راشکی ا، صباغ س.ک، راشکی‌قلعه‌نو ز. بررسی ارتباط پلی‌مورفیسم‌های </span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">Gly972Arg</span></span></strong><strong><span style="font-family:b roya;"><span style="font-size:11.0pt;"> و </span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">Ala512Pro</span></span></strong><strong><span style="font-family:b roya;"><span style="font-size:11.0pt;"> در ژن </span></span></strong><strong><em><span dir="LTR"><span style="font-size:9.0pt;">IRS1</span></span></em></strong><strong><span style="font-family:b roya;"><span style="font-size:11.0pt;"> با بیماری دیابت نوع 2 در استان سیستان و بلوچستان. مجله دانشگاه علوم پزشکی جیرفت، پاییز و زمستان 1397؛ 5 (2)2: 56- 66.</span></span></strong> <div align="center" style="text-align:center;"> <hr align="center" noshade="noshade" size="3" style="color:#00B0F0;" width="98%" ></div> <br> &nbsp; <div style="text-align: center;"><span style="font-size:14px;"><span style="font-family:times new roman;"><span style="color:#0000CD;"><span style="font-size:16px;"><strong>Investigating the Association of Gly972Arg and Ala512Pro Polymorphism of IRS1 gene<br> with Type 2 Diabetes in Sistan and Baluchistan Province</strong></span></span></span></span><br> &nbsp;</div> <div style="text-align: justify;"><span style="font-size:14px;"><span style="font-family:times new roman;"><strong>Ali Islami (MSc)¹, Ahmad Rashki (PhD)^2*, Seyyed Kazem Sabagh (PhD)³, Zahra Rashki Ghaleno (PhD)⁴</strong></span></span></div> <div style="text-align: center;"><span style="font-size:14px;"><span style="font-family:times new roman;"><em>¹</em><em>MSc Student in Genetics, Department of Biology, Faculty of Science, Zabol University, Zabol, Iran</em><br> <em>²</em><em>Associate Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Zabol University, Zabol, Iran</em><br> <em>³</em><em>Associate Professor, Department of Biology, Faculty of Science, Yazd University, Yazd, Iran</em><br> <em>⁴Assistant Professor of Microbiology, School of Medicine, Zabol University of Medical Sciences</em></span></span><br> <br> <span style="font-family:times new roman;"><span style="color:#0000CD;"><strong>Received: </strong></span>12 Oct. 2018&nbsp; <span style="color:#0000CD;"><strong>Accepted: </strong></span>12 Jan. 2019</span><br> &nbsp;</div> <div style="text-align: justify;"><span style="font-size:14px;"><span style="font-family:times new roman;"><span style="color:#0000CD;"><strong>Introduction<span dir="RTL">:</span></strong> </span>Type 2 diabetes is the most common type of diabetes and accounts for 90% of all diagnosed cases of diabetes. The <em>IRS1</em> gene (insulin receptor -1) acts as a mediator between insulin receptors and a complex network of messenger molecules in the muscle, liver and adipose tissue, and therefore may be an appropriate candidate for diabetes type 2. The aim of this study was to identify the association between known <em>IRS1</em> polymorphism (Gly972Arg and Ala512Pro) in a sample of type 2 diabetic patients compared with healthy subject.<br> <span style="color:#0000CD;"><strong>Methods:</strong></span> In this case-control study, a total of 100 patients with type 2 diabetes and 100 healthy individuals were enrolled to study. DNA extraction was performed using phenol-chloroform-protinase K combination method. Determination of genotype of individuals was done by RFLP-PCR methods. The statistical analysis of the results was done using Chi-square test.<br> <span style="color:#0000CD;"><strong>Results</strong>: </span>The results showed that the frequency of GG, GA and AA genotypes for polymorphism (Gly972Arg) rs1801278 was 82, 18 and 0 in patients and 85, 15 and 0 in healthy subjects. The results of statistical analysis showed that there was no significant difference between allelic and genotype frequency of Gly972Arg in patient with healthy controls. (P= 0.61)<span dir="RTL">.</span> Also, Ala512Pro polymorphism was not found in Sistan and Baluchistan population.<br> <span style="color:#0000CD;"><strong>Conclusion:</strong></span> The statistical analysis of the results showed that the alleles of these two polymorphisms from the <em>IRS1</em> gene in the Sistan and Baluchistan population of Iran were not associated with the risk of type 2 diseases.</span></span><br> <br> &nbsp; <div align="center" style="margin-left:21.3pt;"> <hr align="center" noshade="noshade" size="2" width="100%" ></div> <span style="font-size:14px;"><span style="font-family:times new roman;"><em><span style="background-color:#00FFFF;">►</span></em><strong><span style="background-color:#00FFFF;">Please cite this article as follows:</span></strong><br> Islami A, Rashki A, Sabagh K, Rashki Ghaleno Z. Investigating the <a href="https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&cad=rja&uact=8&ved=0ahUKEwj166qcgfjaAhXJslQKHV3YBqQQFggnMAA&url=https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F19716569&usg=AOvVaw15fY2fcuXo5MO02khvU2li">Association of Gly972Arg and Ala512Pro polymorphism of <em>IRS1</em> gene with type 2</a> diabetes in Sistan and Baluchistan Province. Journal of Jiroft University of Medical Sciences<span style="font-size:12px;">.</span> 2018; 1 (3): 56-66.</span></span> <div align="center"> <hr align="center" noshade="noshade" size="2" width="100%" ></div> &nbsp;<br> <br> &nbsp;</div> بیماری دیابت نوع 2, پلی مورفیسم, ژن IRS1 Type 2 diabetes, SNP, IRS1 Gene 56 66 http://journal.jmu.ac.ir/browse.php?a_code=A-10-1-35&slc_lang=fa&sid=1 علی اسلامی 10031947532846002390 10031947532846002390 No احمد راشکی ah_rashki@usal.es 10031947532846002391 10031947532846002391 Yes سیدکاظم صباغ 10031947532846002392 10031947532846002392 No زهرا راشکی‌قلعه‌نو 10031947532846002393 10031947532846002393 No