Volume 9, Issue 1 (5-2022)                   J Jiroft Univ Med Sci 2022, 9(1): 875-885 | Back to browse issues page

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Kia H, Peeri M, Delfan M. The Effect of High Intensity Interval Training with Curcumin Supplementation on The Genes Expression of Bax, Bcl-2 and Caspase-3 in Cardiomyocytes of Type 2 Diabetic Rats. J Jiroft Univ Med Sci 2022; 9 (1) :875-885
URL: http://journal.jmu.ac.ir/article-1-571-en.html
1- . Phd in Sport PhysiologDepartment of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
2- Professor, Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran , m.peeri@iauctb.ac.ir
3- Assistant Professor Department of Exercise Physiology Department of Exercise Physiology, Faculty of Sport sciences, Alzahra University, Tehran, Iran
Abstract:   (425 Views)
Introduction: Gene mutations in diabetic patients is a major cause of apoptosis in myocytes. Optimal volume training with curcumin intake improves cardiomyopathy in diabetic patients. The purpose of this study was to investigate the effects of high intensity interval training with curcumin supplement on the gene expression of Bax, Bcl-2 and Caspase-3 in the cardiomyocytes of rats in type 2 diabetes.
 Materials and Methods: The present study adopted an experimental design in which 40 male diabetic rats were divided into 5 groups of 8; High intensity interval training (HIIT), High intensity interval training+ curcumin (S+HIIT), Diabetic Control+curcumin (S+DC), Diabetic Control (DC), Normal Control (NC). After inducing diabetes, Curcumin was gavaged in all Groups, excluding Normal Control and Diabetic Control Groups, With two hundred mg/kg of Dosage of body weight five days a week for four weeks. 24 hours after the last workout training and recovery session, the subjects were sacrificed and their left ventricle was extracted. Glucose oxidase was used to measure glucose in plasma using ELISA method to measure insulin levels and HOMA-IR method was used to measure insulin resistance index. To determine the expression of Bax, Bcl-2 and Caspase-3 genes, PCR-Real time method and group comparison were used by Two-way ANOVA test at alpha level of 0.05.
Results: The decrease in Bax gene expression in S+HIIT group compared to the DC (p<0.001), S+DC (p<0.01), and HIIT group was significant respectively compared to the DC and S+DS groups (p<0.05) and (p<0.001). The increased in Bcl-2 gene expression in S+HIIT group respectively compared to the DC (p<0.05) and S+DC (p<0.05) and in HIIT group was significant recpectively compared to the DC and S+DC groups (p<0.001) and (p<0.05). The decrease in Caspase-3 gene expression in S+HIIT group compared to the HIIT (p<0.05), DC (p<0.05), S+DC groups (p<0.05) and in the HIIT group was significant compared to the DC (p<0.01) and S+DC groups (p<0.01).
Conclusion: The results showed that high intensity interval training with curcumin supplementation reduced Bax and Caspase-3 genes. Moreover, increasing the gene expression of Bcl-2 due to high intensity interval training with curcumin supplementation can possibly improve diabetic apoptosis in myocardial. The combination of exercise with curcumin supplementation and the interactive role of these two factors may have led to modulations in apoptosis-related genes.
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Type of Study: Research | Subject: Medical Sciences / Physiology
Received: 2022/01/8 | Accepted: 2022/05/15 | Published: 2022/05/31

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