Volume 5, Issue 2 (3-2019)                   J Jiroft Univ Med Sci 2019, 5(2): 56-66 | Back to browse issues page

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Investigating the Association of Gly972Arg and Ala512Pro Polymorphism of IRS1 gene with Type 2 Diabetes in Sistan and Baluchistan Province. J Jiroft Univ Med Sci 2019; 5 (2) :56-66
URL: http://journal.jmu.ac.ir/article-1-239-en.html
Abstract:   (2503 Views)
Investigating the Association of Gly972Arg and Ala512Pro Polymorphism of IRS1 gene
with Type 2 Diabetes in Sistan and Baluchistan Province

 
Ali Islami (MSc)1, Ahmad Rashki (PhD)2*, Seyyed Kazem Sabagh (PhD)3, Zahra Rashki Ghaleno (PhD)4
1MSc Student in Genetics, Department of Biology, Faculty of Science, Zabol University, Zabol, Iran
2Associate Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Zabol University, Zabol, Iran
3Associate Professor, Department of Biology, Faculty of Science, Yazd University, Yazd, Iran
4Assistant Professor of Microbiology, School of Medicine, Zabol University of Medical Sciences


Received: 12 Oct. 2018  Accepted: 12 Jan. 2019
 
Introduction: Type 2 diabetes is the most common type of diabetes and accounts for 90% of all diagnosed cases of diabetes. The IRS1 gene (insulin receptor -1) acts as a mediator between insulin receptors and a complex network of messenger molecules in the muscle, liver and adipose tissue, and therefore may be an appropriate candidate for diabetes type 2. The aim of this study was to identify the association between known IRS1 polymorphism (Gly972Arg and Ala512Pro) in a sample of type 2 diabetic patients compared with healthy subject.
Methods: In this case-control study, a total of 100 patients with type 2 diabetes and 100 healthy individuals were enrolled to study. DNA extraction was performed using phenol-chloroform-protinase K combination method. Determination of genotype of individuals was done by RFLP-PCR methods. The statistical analysis of the results was done using Chi-square test.
Results: The results showed that the frequency of GG, GA and AA genotypes for polymorphism (Gly972Arg) rs1801278 was 82, 18 and 0 in patients and 85, 15 and 0 in healthy subjects. The results of statistical analysis showed that there was no significant difference between allelic and genotype frequency of Gly972Arg in patient with healthy controls. (P= 0.61). Also, Ala512Pro polymorphism was not found in Sistan and Baluchistan population.
Conclusion: The statistical analysis of the results showed that the alleles of these two polymorphisms from the IRS1 gene in the Sistan and Baluchistan population of Iran were not associated with the risk of type 2 diseases.


 

Please cite this article as follows:
Islami A, Rashki A, Sabagh K, Rashki Ghaleno Z. Investigating the Association of Gly972Arg and Ala512Pro polymorphism of IRS1 gene with type 2 diabetes in Sistan and Baluchistan Province. Journal of Jiroft University of Medical Sciences. 2018; 1 (3): 56-66.

 

 
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Type of Study: Research | Subject: Medical Sciences / Medical Genetics
Received: 2019/02/10 | Accepted: 2019/02/10 | Published: 2019/02/10

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